ABSTRACT
Objective To explore the expression level of OTUB1 and its clinical significance in breast cancer.Methods Immunohistochemistry was used to detect the expression level of OTUB1 in 78 cases of breast cancer tissues and 30 cases of normal breast tissue adjacent to carcinoma,and the relationships between OTUB1 and the clinical pathological features of breast cancer were analyzed.Results The positive expression rate of OTUB1 in breast cancer tissues [66.7％ (52/78)] was significantly higher than that in adjacent normal breast tissues [30.0％ (9/30)],with a statistically significant difference (x2 =11.851,P =0.001).OTUB1 expression level was related to the lymph node metastasis (x2 =5.029,P =0.025),postoperative TNM staging (x2 =4.478,P =0.034),expression of human epidermal growth factor receptor-2 (HER-2) (x2 =8.775,P =0.003),expression of P53 (x2 =4.708,P =0.030),expression of estrogen receptor (ER) (x2 =10.364,P =0.001) and molecular subtypes (x2 =10.934,P =0.012).However,OTUB1 expression level in breast cancer was not related to the age (x2 =2.194,P =0.139),menopausal status (x2 =1.843,P =0.175),tumor size (x2 =0.643,P =0.423),histological grade (x2 =3.580,P =0.167),expression of progestin receptor (PR) (x2 =3.371,P =0.066) and expression of Ki-67 (x2 =1.345,P =0.246).Conclusion OTUB1 expression level increases in breast cancer,which is associated with the lymph node metastasis,TNM staging,expressions of HER-2,P53,ER and molecular subtypes of breast cancer.It suggests that the expression of OTUB1 is related to the progression and metastasis of breast cancer.
ABSTRACT
Objective To explore the expression level of OTUB1 and its clinical significance in breast cancer.Methods Immunohistochemistry was used to detect the expression level of OTUB1 in 78 cases of breast cancer tissues and 30 cases of normal breast tissue adjacent to carcinoma,and the relationships between OTUB1 and the clinical pathological features of breast cancer were analyzed.Results The positive expression rate of OTUB1 in breast cancer tissues [66.7％ (52/78)] was significantly higher than that in adjacent normal breast tissues [30.0％ (9/30)],with a statistically significant difference (x2 =11.851,P =0.001).OTUB1 expression level was related to the lymph node metastasis (x2 =5.029,P =0.025),postoperative TNM staging (x2 =4.478,P =0.034),expression of human epidermal growth factor receptor-2 (HER-2) (x2 =8.775,P =0.003),expression of P53 (x2 =4.708,P =0.030),expression of estrogen receptor (ER) (x2 =10.364,P =0.001) and molecular subtypes (x2 =10.934,P =0.012).However,OTUB1 expression level in breast cancer was not related to the age (x2 =2.194,P =0.139),menopausal status (x2 =1.843,P =0.175),tumor size (x2 =0.643,P =0.423),histological grade (x2 =3.580,P =0.167),expression of progestin receptor (PR) (x2 =3.371,P =0.066) and expression of Ki-67 (x2 =1.345,P =0.246).Conclusion OTUB1 expression level increases in breast cancer,which is associated with the lymph node metastasis,TNM staging,expressions of HER-2,P53,ER and molecular subtypes of breast cancer.It suggests that the expression of OTUB1 is related to the progression and metastasis of breast cancer.
ABSTRACT
Objective To evaluate the bone repair capacity ofpolylactic acid-polyglycolic acid copolymer (PLGA)/collagen type Ⅰ (CoI) microspheres combined with BMSCs after being injected in intertrochanteric bone defect of osteoporotic female rats.Methods Prepared PLGA microspheres.The microspheres were coated with Col.BMSCs of the third passage were cultured with PLGA/CoI microspheres.Forty 3-month-old female SD rats were ovariectomized to establish osteoporotic animal models.The osteoporotic rats were randomly divided into 5 groups,including SHAM group,PBS group,Cell group,MS group and Cell+ MS group.There were 8 rats in each group.Different material was injected into the intertrochanteric bone defect site which was made with electric drill.Four rats of each group were sacrificed at 1 month and 3 months post-operation.The fenora were taken to measure the intertrochanteric bone mineral density (BMD) with DEXA and evaluate trabecular stucture with Micro CT.Results After 7 days of coculture,BMSCs seeded on PLGA/CoI microspheres had nice adherance and proliferation.There was no difference of BMC and BMD among all groups at 1 month post-operation.Tb.Th of Cell+MS group was higher than that of PBS group and MS group at 1 month post-operation.％Tb.Ar of Cell+MS group was higher than that of Cell group and MS group at 1 month post-operation.Tb.Sp of Cell+MS group had a tendence to decrease compared with other groups but there was no statistical difference at 1 month post-operation.After 3 months of operation,the BMC of Cell+MS group had a tendence to increase compared with that of PBS group and MS group but showed no statistical difference.BMD and Tb.Th of Cell+MS group was higher than those of other groups.％Tb.Ar of Cell+MS group was higher than that of SHAM group and PBS group.Tb.Sp of Cell+MS group had a tendence to reduce compared with other groups but showed no statistical difference.Conclusion The bone defect of osteoporotic site can be repaired 1 month after the injection of the PLGA/CoI microspheres combined with BMSCs.The trabecular reconstruction and bone quality of osteoporotic site can be improved 3 months after the injection.
ABSTRACT
Objective To discover the role of MCPH1 in DNA double-strand damage induced by ionizing radiation and its relationship with H2AX in esophageal cancer cell ECA109. Methods ECA109 cancer cells accepted 8 Gy 1 h after irradiation were collected for protein extraction and immunofluorescence then MCPH1 and H2AX protein expression and nuclear foci changes were observed. A stable low expression of H2AX cell lines was established and MCPH1 and H2AX protein expression and nuclear foci changes induced by ionizing radiation after silence H2AX were detected. Results (1)A stable low expression of H2AX cell lines in ECA109 cells was successfully constructed. (2)Ionizing radiation could cause the increase of r-H2AX and MCPH1 protein expression, as the same as nuclear focus increase of r-H2AX and MCPH1. (3)The protein level and nucleus focus of r-H2AX and MCPH1 were significantly reduced in ECA109 after silence H2AX. Conclusion MCPH1 is the part of DNA damage response triggered by ionizing radiation and is located in damage response downstream and can be regulated by H2AX.
ABSTRACT
BACKGROUND:Percutaneous injection of bone cement into the vertebral body can enhance the vertebral strength and vertebral stability, and obtain pain relief. But the bone cement may leakage into the paravertebral vessels during injection, and may back to the right ventricle and flow into the pulmonary artery through vena cave and thus causing pulmonary embolism. OBJECTIVE:To evaluate the relationship between cement paravertebral vascular leakage and pulmonary cement embolism during percutaneous vertebroplasty. METHODS:Total y 134 cases of osteoporotic compression fractures treated with vertebroplasty were retrospectively analyzed. Among them, 23 cases of cement paravertebral vascular leakage were considered as the experimental group, and the 43 cases without cement paravertebral vascular leakage and had the complete imaging data were considered as the control group. The spine and chest X-ray films were taken before and after vertebroplasty to detect whether there were cement paravertebral vascular leakage and pulmonary cement embolism in the patients of two groups. RESULTS AND CONCLUSION:Among the 134 patients, 23 patients had cement paravertebral vascular leakage, and accounted for 17.2%. Among the 23 patients with cement paravertebral vascular leakage, three cases had pulmonary cement embolism without chest symptoms, and accounted for 13%. No pulmonary cement embolism occurred in the 43 patients without paravertebral vascular leakage of the control group, and there was no significant difference between two groups (P=0.039). Although the patients with cement pulmonary embolism remained asymptomatic, pulmonary cement embolism remained possible if cement paravertebral vascular leakage was detected during vertebroplasty.
ABSTRACT
ObjectiveTo analyze the influencing factors and clinical results of radial shortening after operation of intra-articular fractures. MethodsA total of 54 patients with intra-articular fractures of the distal radius treated surgically from January 2003 to January 2008 were followed up and divided into radial shortening group ( ＞ 4 mm) and control group ( ＜ 4 mm) by whether radial shortening was more than 4 mm. Fracture types, comminution and defection degree, age, surgical approach and internal fixation method, whether bone implant in operation, post-operative volar tilting angle and ulnar inclination angle, and wrist function score were investigated and compared between the two groups. ResultsThe incidence of radial shortening in post-operation of intra-articular fractures of the distal radius was 30％.Age and whether bone implant in operation were significantly different between the two groups. Type C fractures were more liable to radial shortening than type B fractures. Operation approach and internal fixation method have no significant influence on the radial shortening. Volar tilting angle and ulnar inclination angle were not significantly different between the two groups and the excellent and good rate of function score was lower in the radial shortening group. ConclusionThere is a high incidence of radial shortening after operation for the intra-articular fractures of the distal radius, which affects the clinical results of operation. Therefore, we should pay more attention to the influencing factors and take corresponding precaution methods during operation.
ABSTRACT
Objective To retrospectively analyze whether the kyphosis exists after removal of the internal fixators for thoracolumabar vertebrae fractures. Methods A total of 18 patients (35-68 years old) with thoracolumabar vertebrae fractures (T11-L2 ) were fixed with short segment pedical screw. The fixators were removed one year postoperatively to observe the changes of the Cobb' s angle and trauma vertebra'height. Results All the patients were followed up for 6-24 months ( average 18.7 months),which showed no intraoperative or postoperative complication, breakage or loosening of the screws. Compared to the Cobb angle and the vertebra height before removal of the internal fixators, the average loss of the Cobb angle was 0.7° and that of the vertebra height was 0.8 mm six months after removal of the fixators, 1.9° and 1.1 mm respectively one year after removal of the fixators, and 2.4° and 1.3 mm respectively two years after removal of the fixators in 16 patients without osteoporosis (P ＞0. 05). Among two patients with osteoporosis, the average loss of the Cobb angle and the vertebra height was 6° and 8°respectively and 3 mm and 5 mm respectively six months after removal of the fixators; 13° and 17° respectively and 5 mm and 7 mm respectively one year after removal of the fixators; 15° and 19° respectively and 6 mm and 7.5 mm two years after removal of the fixators. Conclusions After the internal fixation for thoracolumbar vertebrae burst fractures, kyphosis develops mildly, with insignificant change of the vertebral height. While the kyphosis becomes worse after removal of the fixators for thoracolumbar vertebrae burst fractures in patients with osteoporosis.
ABSTRACT
Objective To investigate the effect of radix paeoniae rubra (RPR) on expressions of p38MAPK, NF-κB and iNOS in ltpopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its protective mechanism. Methods Forty Wistar rats with ALI were divided randomly into five groups: saline control group (Group A) , LPS group (Group B), RPR for treatment group (Group C), RPR for prevention group (Group D) and SB203580 group (Group E). The effects of RPR on protein content and the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), contents of malondidehyde (MDA) and serum NO in lung tissue were observed. Arterial blood was collected for blood gas analysis. Expressions of p38MAPK, NF-κB and iNOS in rat lung tissues were detected. Results Compared with Group A, expressions of p38MAPK, NF-κB and iNOS were significantly increased (P <0.01 orP< 0.05) , the protein content and the ratio of neutrophiles in BALF, contents of MDA and serum NO were significantly higher (P<0.01 or P<0.05) in Groups B, C, D and E. There was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in Groups B, C, D and E (P<0.01 or P<0.05). Compared with Group B, the expressions of p38MAPK, NF-κB and iNOS were significantly lower, and the protein content and the ratio of neutrophiles in BALF, contents of MDA and serum NO were significantly decreased, while the levels of arterial bicarbonate and partial pressure of oxygen were significantly higher in Groups C, D and E (P<0.05 or P<0.01). Compared with Group B, the lunginjury of Groups C, D and E was significantly alleviated, while there was no statistical difference among Groups C, D and E in the indices of lung injury. Conclusion Protective effect of RPR on LPS-induced ALI is closely related to the inhibited expressions of p38MAPK, NF-κB and iNOS.
ABSTRACT
Objective To investigate the relationship between bone marrow micrometastasis of patients with breast cancer and clinical pathological parameters, some molecular markers as well as prognosis.Methods The expression of hMAM mRNA in BM of patients with breast cancer was detected by RT-PCR.The expressions of ER, PR in cancer tissues were detected by immunohistochemical SP method. Results About 38.2 % positive expression rate of hMAM mRNA in 102 patients with stage Ⅰ -Ⅳ breast cancer was found.The expression of hMAM increased more in patients with T2-3 (＞2 cm) tumors than T1 (≤2 cm) (P =0.001)and with stage Ⅱ ,Ⅲ than stage Ⅰ (P =0.001). The expression of hMAM in the BM of breast cancer with grade I was lower than that of grade Ⅱ or Ⅲ (P =0.014). The expression of hMAM in the BM was related to the pathological type (P =0.032) and the axillary lymph node metastasis (P =0.001). The expressions of hMAM in BM were much higher with ER negative in breast cancer tissues (P ＜0.05). There was a correlation between patients with positive expression of hMAM in BM and distant metastasis (P =0.009). Conclusion The micrometastasis in BM is correlated with some clinical pathological parameters and some tumor markers. The patients with positive expression of hMAM in BM have more chances with distant metastasis and poor prognosis. The detection of micrometastasis may be as one of targets to predict the prognosis of breast cancer.
ABSTRACT
Objective To investigate the role of p38 mitogan-activated protein kinase signal transduction pathway in lipopolysaccha-ricle-indaced acute lung injury (ALI) in rats. Methods 48 wistar rats were randomly divided into 3 groups, saline control group (group A), lipopolysaccharide (LPS) group(group B) and SB203580 group(group C). Models of lipepelysaccharide-imluced ALI were used to oh-serve the expression of p38MAPK in rat lung, protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF) , pulmonary MDA content and the activities of serum NO. After LPS dripping for 6 hours, arterial blood was drawn for analysis and lung tissue was detec-ted. Results Compared with those in group A, expression of p38MAPK were markedly increased in group B and C (P<0.01). But in group C, expression of P38MAPK was significantly lower than that in group B (P<0.05). The protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), content of pulmonary MDA and the activities of serum NO in group B, C were significantly higher than those in group A (P<0.01). There was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in group B and C (P<0.01). Compared with those in group B, these indexes of lung injury were significantly lower while the level of arterial bicar-bonate and partial pressure of oxygen was increased in group C(P <0. 05 orP <0. 01). Under light microscope, the pathologic changes in-duced by LPS were significantly attenuated by SB2035g0. Conclusion The activation of P38MAPK play an important role in the mechanism of lipopolysaccharide-induced ALI.
ABSTRACT
ObjectiveTo investigate the effects of pre-infusion of hypertonic sodium chloride hydroxyethyl starch 40(HH40)injection in prevention for transurethral electroresection of the prostate syndrome(Tuns).MethodsSixty patients with benign prostate hyperplasia(ASA)grades Ⅱ toⅢwere recruited in the study and randomly divided into two groups,one injected intravenously with HH40 at dose of 5 ml/kg by drip after sursical operation a8 experiment and the other injcoted with the same volume of Ringer's solution as control Mcan arterial pressure,heart rate,saturation of blood oxygen(SaO2)and central venous pressure were monitored for all the patients.In addition.serum concentrations of sodium,potassium,chloride and serum osmolality were determined before(T0)and 30 min(T30)and 60 min(T60) after operation,respectively,as well as pH valRe and partial pressure of carbon dioxide(PgCO2)in the gastric mucosa were determined by a Tonocap monitor.Occurrence of TURS and use of atropine,ephedrine and rinse solution were documented.Resuits TURS occurred in four patients in the eontrol group during surgical operation,whereas uo TUns occurred in experimental group.Serum sodium concentration was (126.5±13.3)mmoL/L at T60 in control group,significantly lower than that in experimental group(P＜0.05),while intragastric pH value and PgC02 level were(7.26±0.04)and(54±9)mm Hg[(7.2±1.2)kPa],respectively in controls,significantly higher than those in experimental group(P＜0.05).ConclusionsPre-infusion with HH 40 can improve tissue perfusion and prevent effectively from TURS in patients with transurethral electroresection of the prostate.
ABSTRACT
BACKGROUND: The main pathological change of intervertebral disc herniation is that nucleus pulposus protrudes from ruptured annulus fibrosus, thus we can hypothesize that it is possible to prevent disc herniation prior to its protrusion by coagulating it as a whole.OBJECTIVE: To observe the coagulation effects of intervertebral disc nucleus pulposus by alum solution.DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed between September 2002 and April 2003 at Department of Animal Experiment, Peking University First Hospital, Beijing China.MATERIALS: Twenty-six healthy adult hybrid dogs, 9 for in vitro experiment and 17 for in vivo experiment, weighing 16-21kg, with no restrictions on male and female, were obtained from Department of Animal Experiment, Peking University First Hospital, Beijing, China.METHODS: Twenty canine in vitro intervertebral discs obtained from 5 dogs were randomly divided into 4 groups, with 5 discs in each group, and were put into 2.5%, 5%, 10% alum solutions and 0.9% physiological saline, respectively. Effects of disc coagulation were observed after immersing for 1 day and 10 days, respectively. Another 16 in vitro intervertebral discs obtained from 4 dogs, composed of L2/3, L3/4, L4/5, L5/6, were also injected with the above 4 experimental solutions, 0.15ml, respectively. Sixty-eight in vivo intervertebral discs were obtained from 17 dogs and divided into 4 groups: blank control group, physiological saline group, 10% alum solution+one puncture point group, and 10% alum solution+two puncture points group, 17 discs in each group. Harvesting time: 3 days, 2 weeks, 1 month and 3 months postoperation.MAIN OUTCOME MEASURES: Effects of alum solutions on the coagulation of the intervertebral discs and related histological changes were observed and an alum solution of suitable concentration was preliminarily selected. General observation, light microscopic observation and scanning electron microscopic observation were made of the nucleus pulposuses.RESULTS: In the in vitro and in vivo experiments, it was found that physiological saline did not produce the effect of coagulation on the nucleus pulposus, while immersion in the alum solution induced nucleus pulposus coagulation in the in vitro intervertebral discs. Also, as the concentration of the alum solution increased, the volume of the coagulated nucleus pulposus gradually decreased. After alum solution was injected into the in vitro intervertebral discs, no nucleus pulposus coagulation appeared. When the 10% alum solution was injected into the in vivo intervertebral discs, nucleus pulposus coagulation occurred, with the strongest coagulation effect reached at 1 month postoperation. This was manifested in the agglutination reaction centered around the puncture point. When there were 2 puncture points, 2 coagulated lumps might appear. There was an increase in the mesenchymal component of the coagulated nucleus pulposus. Histochemical and scanning electron microscopic examinations confirmed the proliferation of large numbers of collagen fibers in the mesenchyme.CONCLUSION: Alum can promote nucleus pulposus to coagulate around the injection point. This may be related to the increase of collagens and the fibrosis resulting from stimulation of the nucleus pulposus by alum solution.
ABSTRACT
[Objective]To investigate the effect of coagulation of intervertebral disc nucleus pulposus(IDNP) by alum.[Method]In vitro,20 canine intervertebral discs(I D) were divided into 4 groups,and immersed into 2.5%,5%,10% alum solution and normal saline respectively.Another 16 I D also randomized into 4 groups and were injected into I D with corresponding solution.In vivo,6 canine ID were grouped into 4:A.blank control;B.one injection of alum;C.two points injection of alum;D.injection of N.S.Experimental dogs were sacrificed and disc samples were taken at 3 days,2 wks,and 1 month post-injection.Gross observation,histological exam by microscopy and scan electron microscopy(SEM) and histochemical test were performed.[Result]NS did not coagulate the IDNP in vivo and in vitro,while in vivo,coagulation was seen around the injection points,two points with two coagulation blocks,and grew with time culminating on one month after injection,but there was no coagulation appeared after alum injection.In vitro,histological study showed aboundant collagenous fibers gathered in coagulation lock representing manifold function of mesenchyme.[Conclusion]The alum can make the IDNP to be coagulated in vivo,the mechanism of coagulation is the collagenous fiber proliferation and fibrosis due to manifold function of mesenchyme.
ABSTRACT
BACKGROUND: The abnormal apoptosis of intestinal epithelial cells is the main cause of intestinal mucous membrane injury during ischemia reperfusion. Shenfu injection has good therapeutic effect on intestinal mucous membrane injury. OBJECTIVE: To investigate the influence of shenfu injection medication on intestinal apoptotic epithelial number, apoptosis-related caspase-3 and Bcl-2 gene expression, as well as the level of tumor necrosis factor (TNF)in intestinal ischemia reperfusion rat model.DESIGN: Randomized controlled experiment.SETTING:Department of Anesthesia, Renmin Hospital of Wuhan University.MATERIALS:This experiment was carried out in the laboratory of the Department of Anesthesia, Renmin Hospital of Wuhan University, between December 2002 and June 2003. Totally 24 healthy SD rats of clean grade were randomized into blank control group, intestinal ischemia reperfusion (IR) group and shenfu injection group with 8 rats in each group.METHODS: Rats in each group were anesthetized with intraperitoneal injection of ethyl carbamate at dosage of 1mg/kg; then in intestinal IR group and shenfu injection group rats' superior mesenteric artery was occluded with vascular clamp for 1 hour before 2-hour reperfusion,which was not conducted in blank control group. Rats in shenfu injection group were intravenously injected with shenfu injection at 30 minutes before occlusion at dose of 0.02 mL/g, which was replaced by the same volume of physical saline in blank control group and intestinal IR group. The expression of caspase-3 and Bcl-2 protein and cell apoptosis wee detected.MAIN OUTCOME MEASURES: ① Comparison of apoptotic index between groups. ② The expression of caspase-3 and Bcl-2 gene in rat intestinal epithelium. ③ Comparison of TNF content in intestinal mucous membrane homogenate between groups.RESULTS: Totally 24 rats were included in this experiment and all ehtered the result analysis. ①Comparison of apoptotic index between groups:The apoptotic index was obviously lower in shenfu injection group than in intestinal IR group, but higher than in blank control group [(7.75-±1.89)%,(28.25±8.50)%, (4.25-±2.63)%, P ＜ 0.01]. ② The expression of caspase-3 and Bcl-2 gene in rat intestinal epithelium: caspase-3 expression was lower in shenfu injection group than in intestinal IR group, but higher than in blank control group [(0.211 6±0.087 5), (0.354 7±0.077 8), (0.194 1±0.057 4) A,P ＜ 0.01, P ＞ 0.05]; Bcl-2 expression was remarkably higher in intestinal IR group than in blank control group (P ＜ 0.05), but obviously reduced in shenfu injection group compared to intestinal IR group (P ＜ 0.01). ③ TNF content of intestinal mucous membranehomogenate in each group: TNF content was remarkably higher in intestinal IR group than in blank control group and shenfu injection group [(189.7±56.3), (38.6±10.4), (47.5±l8.7)mg/L,P ＜ 0.01], and basically the same in shenfu inj~tion group and blank control group (P ＞ 0.05).CONCLUSION: Shenfu injection can suppress intestinal epithelial apoptosis by reducing TNF content and caspase-3 e.pression as well as upregulating the expression of Bcl-2 gene during ischemia reperfusion, thereby attenuating ischemia reperfusion injury of intestinal mucous membrane.
ABSTRACT
AIM: To explore protective and therapeutic effects of the shenfu injection (SF) against renal ischemia reperfusionand and its possible mechanisms by studying the impact of SF on P38 mitogen activated protein kinase (P38MAPK), tumor necrosis factor alpha (TNF-?) and intercellular adhensive molecule-1 (ICAM-1). METHODS: 36 male SD rats were randomly divided into control group, ischemia reperfusion (IR) group and SF group. Rats were subjected to left renal pedicle occlusion followed by reperfusion with contralateral nephrectomy. The expression of P38MAPK and ICAM-1 were evaluated by Imunohistochemistry. The expression of TNF-? in the kidney tissue and plasma were analyzed by enzyme-linked immunoadsordent assay (ELISA). RESULTS: The expression of P38MAPK and ICAM-1, concentration of TNF-? in IR group were higher than that in the control group (P
ABSTRACT
Objective: To evaluate the effect of severe rigid kyphotic scoliosis treated with posterior spinal osteotomy Methods: A total of 11 cases(average 11.2 years) of severe rigid kyphotic scoliosis treated with posterior spinal osteotomy from Mar.2005 to Sept. 2006 were retrospective. Of those cases, 3 were of neurofibromatosis, 5 of congenital scoliosis, 1 of poliomyelitis and 2of idiopathic scoliosis. The flexibility of all patients was less than 25%. They had an average follow-up period of 1.3 years (0.5-2 years) Results: There were 3 cases of one segment posterior wedge osteotomy, 2 of two segment posterior wedge osteotomy and 2 of three segment wedge osteotomy. Transpedicular osteotomy was performed in 2 cases, and the last 2 cases encountered vertebral resection and posterior column removed. The average kyphotic angle was 108 degrees (ranging from 87 to 135 degrees) and the average scoliosis angle was 97 degrees (ranging from 65 to 135 degrees) before operation. After operation the average kephotic angle was corrected to 49 degrees (the correction rate was 55%) and the average scoliosis angle was corrected to 37 degrees(the correction rate was 66%) . The height rose up by an average of 4.7 cm. No neurological complication happened in all the 11 cases. Conclusion: It is an effective method that severe rigid kyphotic spinal scoliosis is treated with posterior osteotomy.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cervical spine instability on sympathetic cervical spondylosis.</p><p><b>METHODS</b>Twelve patients with cervical spondylosis showing sympathetic symptoms from 1992 to 2 000 were evaluated for cervical spine instability, immobilization of collar brace, MRI and surgical results.</p><p><b>RESULTS</b>Radiologic evaluation cervical instability was observed radiologically in 7 of the 12 cases. Collar brace was obviously effective in 5 of the 12 cases and slightly effective in 7 cases. MRI showed abnormal signal for instability of the cervical spine in 7 cases. Surgery was markedly effective in 7 cases and moderately effective in 4 cases.</p><p><b>CONCLUSION</b>Cervical spine instability was an important factor for sympathetic cervical spondylosis, which can be improved effectively by surgery.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Autonomic Nervous System Diseases , Cervical Vertebrae , Magnetic Resonance Imaging , Spinal Osteophytosis , Diagnosis , General SurgeryABSTRACT
Objective To study the clinical features and treatment of the patients with Takayasu's arteritis (TA) with cerebral infarction.Methods Retrospective analysis was done in 14 TA patients with cerebral infarction from 1998 to 2003.Results The patients with TA complicated with cerebral infarction accounted for 10.6%(14/132) of all the hospitalized patients with TA.The time from cerebral infarction to the symptom onset of TA was 1 month to 15 years.In all the cases,there were more than 3 branches stenosis or occlusion of aortic arch and hemiparalysis.Basal ganglion was the most frequently involved infarction area.Corticosteroid and cytotoxic drugs were effective therapy.Conclusion The cases of TA with cerebral infarction have extensive arterial lesions.We must pay attention to it to avoid misdiagnosis.The key point of treatment is to control the primary disease.
ABSTRACT
Objective: To expeore the effectiveress of partial splenectomy for treating splenic benign tumor.Methods: The 16 patients of splenic benign tumor were performed partial splenectomy. Six patients in all were adopted partial splenectomy plus great omentum encapsulation. Ten patients were used partial splenectomy plus glue spray.The postoperative complication was analyzed. Results: Only 1 patient suffered from left subphrenic abscess, one patient took place partial splenic necrosis, the rest had't complication. The average hospieal stay was 12 days.Conclusion: The partial splenectomy has few complications and the hospital stay is much short.This procedure is safe for treating splenic benign tumor.
ABSTRACT
Objective To investigate the effect of propofol on expression of cyclinD1 and apoptosis in spinal cord neurons induced by ischemia-reperfusion (I/R) .Methods Sixty male Wistar rats weighing 200-250 g were randomly divided into 2 groups ( n = 30 each): group A I/R and group B propofol + I/R. The animals were anesthetized with 7% chloral hydrate 6 ml?kg-1. The abdomen was opened and the abdominal aorta was clamped distal to the left renal artery for 20 min. The aortic cross clamp was then released to allow reperfusion. In propofol group propofol 100 mg?kg-1 was administered intraperitoneally (IP) during the operation. Neurologic function was assessed using Taylor scale (0 = unable to move hind limbs, 4 = normal function) at 6 h after operation and on postoperative day 1, 2, 3, 7 (n = 6 at each time point) . The animals were killed after neurologic function evaluation and the spinal cord was removed for microscopic examination, detection of apoptosis in the spinal cord neurons ( TUNEL) and determination of cyclinD1 expression (immuno-histochemistry) . Results The histo-pathological damage to the neurons was significantly lighter, the neurological function better and the apoptotic index and the cyclinD1 expression were significantly lower in propofol group than in I/R group. Conclusion Propofol protects spinal cord against I/R injury by reducing neuronal apoptosis through down-regulation of cyclinD1 expression.